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September 2002, Volume 8 No. 2
ARTICLE 4
Antioxidant Activity of Selected Commercial Seaweeds
Amin Ismail & Tan Siew Hong
Department of Nutrition and Health Sciences, Faculty of Medicine and
Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia
ABSTRACT
This study aims to evaluate the antioxidant activity (total
antioxidant and free radical scavenging activities) of seaweeds
commercially available in the Malaysian supermarket. Four types of
seaweeds namely Nori (Porphyra sp.), Kumbu (Laminaria
sp.), Wakame (Undaria sp.) and Hijiki (Hijikia sp.)
were used in the study. The extracts were prepared with water and
ethanol, respectively. The b-carotene bleaching and
1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used to determine
antioxidant properties of seaweeds by measuring the decrease in
absorbance at 470 and 517 nm. In water extract, Kumbu showed the
highest total antioxidant activity of 63% compared with other
samples. Kumbu, Nori and Hijiki exhibited higher radical scavenging
activity than Wakame when extracted with water. Wakame exhibited
the highest antioxidant and free radical scavenging activities in
ethanolic extract with 58% and EC50 = 0.42 mg/ml respectively. The
results of ANOVA analysis show significant differences (p<0.05) in
the means of total antioxidant and free radical scavenging
activities of the seaweeds. The results showed that processed
commercial seaweeds exhibited varying degrees of antioxidant
properties.
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