Mal J Nutr 22(2): 267 - 278, 2016

Glucose Lowering Effect of Horseradish Tree (Moringa oleifera Lam. Leaf Decoction in Alloxan-Induced Diabetic Mice
Joan R Ilagan1, Wilma A Hurtada1, Aimee Sheree A Barrion1, Maria Amelita C Estacio2 & Erlinda I Dizon3

1Institute of Human Nutrition and Food, College of Human Ecology,University of the Philippines, College, Los Baños, Laguna, Philippines 4031 2 College of Veterinary Medicine, University of the Philippines, College, Los Baños, Laguna, Philippines 4031 3 Food Science Cluster, College of Agriculture, University of the Philippines, College, Los Baños, Laguna, Philippines 4031


Introduction: The study was conducted to determine the glucose lowering effect of horseradish tree leaf decoction (HTLD) in alloxan-induced diabetic mice. Methods: The leaves of the Institute of Plant Breeding Moringa 3 (IPBM3) strain of horseradish tree were used in this study. The median effective dose (ED50) of IPBM3 HTLD was determined using normal Institute of Cancer Research (ICR) mice for 14 days and the computed ED50 was administered once, twice and thrice per day for 28 days via gavage in alloxan-induced diabetic mice. Fasting blood glucose level (FBGL), body weight, feed intake and water intake were determined weekly. Thin layer chromatography and in vitro glucose lowering activity experiments were also done to determine the major polyphenol found in IPBM3 HTL with glucose lowering activity. Results: Administration of the ED50, 417 mg/20 mL/ kgbw, once, twice and thrice per day for 28 days significantly reduced the mean FBGL of diabetic mice by more than 50% and the reduction was statistically comparable with the metformin-treated group. Mean body weights and feed intakes were normal and statistically comparable for all groups. Mean water intakes were slightly higher than in the non-diabetic control group. The major polyphenol in IPBM3 HTLD was found to be quercetin. The in vitro glucose lowering activity experiment showed that both petroleum ether and water extracts of IPBM3 HTL have glucose lowering activity similar to standard quercetin. Conclusion: The present results show that IPBM3 HTLD possesses glucose lowering activity and offers potential for use in the management of diabetes mellitus.

Keywords: Blood glucose, diabetes mellitus, horseradish tree leaves, phenolics, quercetin

Download full article

March 1995, Vol1 No.1
September 1995, Vol1 No.2
March 1996, Vol2 No.1
September 1996, Vol2 No.2
March 1997, Vol3 No.1
September 1997, Vol3 No.2
December 1998, Vol4 No.1&2
December 1999, Vol5 No.1&2
March 2000, Vol6 No.1
September 2000, Vol6 No.2
Mar/Sept 2001, Vol7 No.1&2
March 2002, Vol8, No.1
September 2002, Vol8, No.2
March 2003, Vol9 No.1
September 2003, Vol9 No.2
March 2004, Vol10 No.1
September 2004, Vol10 No. 2
2005, Vol 11 No.1
2005, Vo l11 No.2
2006, Vol 12 No.1
2006, Vol 12 No.2
2007, Vol 13 No.1
2007, Vol 13 No.2
March 2008, Vol 14 No.1
2008, Vol 14 No.2
2009, Vol 15 No.1
2009, Vol 15 No.2
2010, Vol 16(1)

2010, Vol 16(2)

2010, Vol 16(3)

2011, Vol 17(1)

2011, Vol 17(2)

2011, Vol 17(3)

2012, Vol 18(1)

2012, Vol 18(2)

2012, Vol 18(3)

2013, Vol 19(1)

2013, Vol 19(2)

2013, Vol 19(3)

2014, Vol 20(1)

2014, Vol 20(2)

2014, Vol 20(3)

2015, Vol 21(1)

2015, Vol 21(2)

2015, Vol 21(3)

2016, Vol 22(1)

2016, Vol 22(2)

2016, Vol 22 Supplement

2016, Vol 22(3)

2017, Vol 23(1)

2017, Vol 23(2)