Mal J Nutr 22(1): 137 - 151, 2016

Silymarin Supplementation Attenuation of the Lipopolysaccharide-Induced Metabolic Changes in Protein Malnourished Rats
Adel A Alhamdan

Health Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia

ABSTRACT

Introduction: Protein malnutrition increases the sensitivity to septic shock by impairing antioxidation and immune response. Based on the potent antioxidant effects of silymarin, the putative protective role of silymarin against sepsis-induced oxidative damage in protein malnourished rats was investigated. Methods: Adult male Wistar rats were subjected to protein malnutrition via a low-protein diet (8% protein), with and without silymarin supplementation (30 mg/kg/day) for four weeks, and compared to a control group on a 18% protein diet At end of the experiment, the animals received intraperitoneal injections (i.p.) of 0.1 mg/ kg lipopolysaccharide (LPS) derived from Esdieridtia coil, and decapitation 24 h later. Albumin, C-reactive protein (CRP), total protein (TP), alkaline phosphates (A/CP ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), tumor necrosis factor-a (TNF-a), interleultine-lp (IL-1p) and interleukine-6 (IL-6) levels were measured in serum. In the lungs and liver, thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) concentrations, superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transferase (GST) activities were measured. Results: oxidative and inflammatory processes were significantly increased by the LPS injection and these changes were to a greater extent in the low-protein dietary group compared to control group. Silymarin supplementation in both dietary groups showed marked inhibition in these activities, although the affect was more prominent in the control group compared to the rats fed a low-protein diet. Conclusions: The study showed that silymarin protected against the impairment of antioxidation and immune response in protein malnourished rats, particularly in septic shock conditions.

Antioxidant, cytokines, endotoxin, protein malnutrition, silymarin

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