Mal J Nutr 20(2): 271 - 282, 2014

Anti-Inflammatory Activities of Digested Green Curry Paste in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients
Sirikanokvilai P1, Kriengsinyos W1, Nantiruj K2, Muangnoi C1, Chingsuwanroj P1, Praengam K1 & Tuntipopipat S 1*

ABSTRACT

Introduction: Several individual spices have been shown to have antiinflammatory activity. However, the anti-inflammatory activities of mixed spices consumed as components of habitual diets are limited. We aimed to assess the anti-inflammatory activities of ‘Green curry paste ‘(GCP) consisting of nine different spices in cell culture models.
Methods: Non-toxic diluted bioaccessible fraction generated by simulated digestion of GCP was added to the apical compartment of Caco-2 cells grown on bicameral inserts for 4 h. Basolateral media (bioavailable fraction) was added to cultures of peripheral blood mononuclear cells (PBMC) from 12 patients with rheumatoid arthritis in the absence or presence of lipopolysaccharide (LPS). Cytokines in culture media were quantified by ELISA. Caco-2 cells also were activated with IL-1ß following exposure to the bioaccessible fraction of GCP to determine its effect on chemokine expression.
Results: Spontaneous and LPS-induced secretions of IL-6 were significantly decreased when PBMC were exposed to the basolateral medium from Caco-2 cell cultures incubated with the bioaccessible fraction of GCP compared to control cultures. In a similar procedure, secretions of TNF-a and IL-8 were also decreased, but the changes were not significantly different from the controls. Pre-treatment of Caco-2 cells with the bioaccessible fraction of GCP also significantly suppressed IL-1ß -induced expression of IL-8 (62% protein and 63% mRNA) and MCP-1 (47% protein and 51% mRNA) (p<0.05).
Conclusion: These results suggest the use of GCP as a food component towards attenuating the severity of inflammatory conditions.

Keywords: Anti-inflammatory activity, Caco-2 human intestinal cells, green curry paste, peripheral blood mononuclear cells

Download full article

March 1995, Vol1 No.1
September 1995, Vol1 No.2
March 1996, Vol2 No.1
September 1996, Vol2 No.2
March 1997, Vol3 No.1
September 1997, Vol3 No.2
December 1998, Vol4 No.1&2
December 1999, Vol5 No.1&2
March 2000, Vol6 No.1
September 2000, Vol6 No.2
Mar/Sept 2001, Vol7 No.1&2
March 2002, Vol8, No.1
September 2002, Vol8, No.2
March 2003, Vol9 No.1
September 2003, Vol9 No.2
March 2004, Vol10 No.1
September 2004, Vol10 No. 2
2005, Vol 11 No.1
2005, Vo l11 No.2
2006, Vol 12 No.1
2006, Vol 12 No.2
2007, Vol 13 No.1
2007, Vol 13 No.2
March 2008, Vol 14 No.1
2008, Vol 14 No.2
2009, Vol 15 No.1
2009, Vol 15 No.2
2010, Vol 16(1)

2010, Vol 16(2)

2010, Vol 16(3)

2011, Vol 17(1)

2011, Vol 17(2)

2011, Vol 17(3)

2012, Vol 18(1)

2012, Vol 18(2)

2012, Vol 18(3)

2013, Vol 19(1)

2013, Vol 19(2)

2013, Vol 19(3)

2014, Vol 20(1)

2014, Vol 20(2)

2014, Vol 20(3)

2015, Vol 21(1)

2015, Vol 21(2)

2015, Vol 21(3)

2016, Vol 22(1)

2016, Vol 22(2)

2016, Vol 22 Supplement

2016, Vol 22(3)

2017, Vol 23(1)

2017, Vol 23(2)